Pumping tests conducted on wells intersecting karst heterogeneities such as the conduit network are difficult to interpret. Nevertheless, this case can be solved by assimilating the horizontal karst conduit to a finite conductivity vertical fracture. In this case, several flow patterns corresponding to the respective contributions of karst subsystems (fractured matrix, small conduits, and main karst drainage network) can be identified on the diagnostic plot of the drawdown derivative. This is illustrated on two examples from Mediterranean karst systems in southern France. A pumping test on a well intersecting the main karst drainage network of the Cent-Fonts karst system shows (i) a preliminary contribution of the karst conduit storage capacity followed by (ii) linear flows into the fractured matrix. A pumping test on a well intersecting a small karst conduit of the Corbières karst system shows the existence of (i) bi-linear flow within both the karst conduit and the fractured matrix at early times, followed by (ii) radial flows within the fractured matrix and (iii) finally the contribution of a major karst cavity. The use of diagnostic plots allows identifying the various flow regimes during pumping tests, corresponding to the response of the individual karst aquifer subsystems. This is helpful for improving the understanding of the structure of the karst aquifer and flow exchanges between subsystems.